丁酸梭菌替代金霉素对肉鸡腿肌肉品质和抗氧化功能的影响

丁酸梭菌（Clostridium butyricum）是一种产丁酸等短链脂肪酸的有益菌，添加到饲料中可改善动物的生产性能、提高饲料利用率和机体的抗氧化功能。【目的】本文研究了丁酸梭菌替代金霉素添加到肉鸡日粮中，对肉鸡腿肌肉品质和抗氧化功能的影响。【方法】随机选取1日龄健康艾拔益加（AA）肉仔鸡360只，分为3组，每组8个重复，阴性对照组（CON）饲喂基础日粮；抗生素组（ANT）饲喂添加75 mg/kg金霉素的基础日粮；丁酸梭菌组（CB）饲喂添加500 mg/kg丁酸梭菌（活菌数10⁹ CFU/g）的基础日粮，试验为期42 d。【结果】与CON组相比，CB组肉鸡腿肌重、腿肌肉24 h红度（a^*）、pH_{45 min}、pH_{24 h}、粗蛋白质和粗脂肪的含量显著提高，腿肌肉总抗氧化能力（total-antioxidant capacity,T-AOC）、过氧化氢酶（catalase,CAT）、总超氧化物歧化酶（total superoxide dismutase,T-SOD）和谷胱甘肽过氧化物酶（glutat...

Effect of Clostridium butyricum as a replacement of chlortetracycline on quality and antioxidation of broiler leg muscle

The beneficial Clostridium butyricum,which synthesizes short-chain fatty acids such as butyric acid,can be added to feed to improve animal production,feed utilization efficiency,and the antioxidant ability of animals.Objective] This paper explored effect of C.butyricum as a replacement of chlortetracycline on quality and antioxidant function of broiler leg muscle.Methods] A total of 360 healthy 1-day-old Arbor Acres broilers were randomly selected and classified into three groups,with 8 replicates each group.Negative control group (CON) was fed with basal diet,antibiotics group (ANT) basal diet supplemented with 75 mg/kg chlortetracycline,and C.butyricum group (CB) basal diet supplemented with 500 mg/kg C. butyricum(viable bacteria,10⁹ CFU/g).The experiment lasted 42 days.Results] Compared with CON group,CB significantly increased weight,24h redness (a^*),pH_{45 min},pH_{24 h},crude protein content,and ether extract,decreased 24 h brightness (L^*),drip loss,cooking loss,and malondialdehyde (MDA) content,improved the total-antioxidant capacity (T-AOC),and the activity of catalase (CAT),total superoxide dismutase (T-SOD),and glutathione peroxidase (GPx),and up-regulated the expression of nuclear factor erythroid 2-related factor 2(Nrf2),GPx-1,SOD-1,and SOD-2 of broiler leg muscle (P<0.05).ANT raised the weight and pH_{45 min},reduced the drip loss and MDA content,and up-regulated the expression of Nrf2,GPx-1, and SOD-1 of broiler leg muscle compared with CON group (P<0.05).CB group demonstrated lower drip loss and L^* after 24 h and higher ether extract and CAT activity of broiler leg muscle than the ANT group (P<0.05).Conclusion] C. butyricum can replace chlortetracycline for broiler feed,activate the expression of Nfr2 signaling pathway related genes,and which improves the quality and antioxidation of broiler leg muscle.