Direct Introduction of Fluorescein Isothiocyanate-conjugated Albumin into Intact Macroconidia of Fusarium oxysporum f. sp. lycopersici by Electroporation |
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Authors: | Y. Matsuda H. Toyoda T. Nishiguchi S. Ouchi |
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Affiliation: | Laboratory of Plant Pathology, Faculty of Agriculture, Kinki University, Japan |
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Abstract: | An electroporation procedure for the introduction of fluorescein isothiocyanate-conjugated albumin into intact macroconidia of Fusarium oxysporum f. sp. lycopersici was performed in this paper. FITC-albumin was used to establish an efficient electroporation procedure because its presence in spores could be easily detected by fluorescence microscopy. The uptake of fluorescein isothiocyanate-conjugated albumin into spores was successfully mediated by high voltage electrical pulses at field strength of 10 KV/cm. In electroporation of 106 macroconidia per ml with the present system, 1.2 × 105 spores incorporated the protein, and then 3.1 × 104 normally germinated, elongated vegetative hyphae, and produced spores. |
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