Direct Introduction of Fluorescein Isothiocyanate-conjugated Albumin into Intact Macroconidia of Fusarium oxysporum f. sp. lycopersici by Electroporation

An electroporation procedure for the introduction of fluorescein isothiocyanate-conjugated albumin into intact macroconidia of Fusarium oxysporum f. sp. lycopersici was performed in this paper. FITC-albumin was used to establish an efficient electroporation procedure because its presence in spores could be easily detected by fluorescence microscopy. The uptake of fluorescein isothiocyanate-conjugated albumin into spores was successfully mediated by high voltage electrical pulses at field strength of 10 KV/cm. In electroporation of 10⁶ macroconidia per ml with the present system, 1.2 × 10⁵ spores incorporated the protein, and then 3.1 × 10⁴ normally germinated, elongated vegetative hyphae, and produced spores.