|
|||||
|
|
Imaging of plant microtubules with high resolution scanning electron microscopy |
|
| Authors: | P. A. Vesk D. G. Rayns M. Vesk |
| Affiliation: | (1) Electron Microscope Unit, F09, University of Sydney, 2006 Sydney, NSW, Australia;(2) Present address: Department of Anatomy and Structural Biology, University of Otago, Dunedin, New Zealand |
| Abstract: | Summary High resolution scanning electron microscopy was used to obtain images of cortical microtubules and associated structures in onion root tips. Specimens were prepared using a modified quick-freeze deep-etch technique utilising cytosolic extraction with saponin and conductive staining with osmium.Abbreviations DMSO dimethylsulfoxide - HRSEM high resolution scanning electron microscope/microscopy - MTSB microtubule stabilising buffer - TEM transmission electron microscope/microscopy |
| Keywords: | High resolution scanning electron microscopy Plant microtubules Quick-freeze deep-etch |
| 本文献已被 SpringerLink 等数据库收录！ | |