Ammonium-dependent hydrogen peroxide production by mitochondria |
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Authors: | Grivennikova Vera G Cecchini Gary Vinogradov Andrei D |
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Affiliation: | Department of Biochemistry, School of Biology, Moscow State University, Moscow 119992, Russian Federation. |
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Abstract: | NADH-supported generation of H2O2 by permeabilized rat heart mitochondria was partially prevented by the specific complex I-directed inhibitor, NADH-OH, and was significantly stimulated by ammonium. Ammonium did not affect H2O2 production by complex I in coupled submitochondrial particles. The soluble mitochondrial matrix protein fraction catalyzed NADH-dependent H2O2 production, which was greatly (approximately 10-fold) stimulated by ammonium. We conclude that complex I is not the major contributor to mitochondrial superoxide (hydrogen peroxide) generation and that there are specific ammonium-sensitive NADH:oxygen oxidoreductase(s) in the mitochondrial matrix which are responsible for mitochondrial H2O2 production. |
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