A rapid and hazardous reagent free protocol for genomic DNA extraction suitable for genetic studies in plants |
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Authors: | Simeon O Kotchoni Emma W Gachomo |
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Institution: | (1) Agronomy Department, Purdue University, Lilly Hall, 915 West State Street, West Lafayette, IN 47907, USA;(2) Department of Biology, Rutgers University, Science Building, 315 Penn Street, Camden, NJ 08102, USA |
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Abstract: | Protocols for genomic DNA extraction from plants are generally lengthily, since they require that tissues be ground in liquid
nitrogen, followed by a precipitation step, washing and drying of the DNA pellet, etc. This represents a major challenge especially
when several hundred samples must be screened/analyzed within a working day. There is therefore a need for a rapid and simple
procedure, which will produce DNA quality suitable for various analyses. Here, we describe a time and cost efficient protocol
for genomic DNA isolation from plants suitable for all routine genetic screening/analyses. The protocol is free from hazardous
reagents and therefore safe to be executed by non-specialists. With this protocol more than 100 genomic DNA samples could
manually be extracted within a working day, making it a promising alternative in genetic studies of large-scale genomic screening
projects. |
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Keywords: | Arabidopsis thaliana Genomic DNA PCR Protocol Restriction enzymes |
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