首页 | 本学科首页   官方微博 | 高级检索  
     

长穗偃麦草中E和E1基因组高分子量麦谷蛋白 基因启动子部分序列的进化分析
引用本文:王宏林,喻德跃,王永军,陈受宜,盖钧镒WANG Hong-Lin,YU De-Yue,WANG Yong-Jun,CHEN Shou-Yi,GAI Jun-Yi. 长穗偃麦草中E和E1基因组高分子量麦谷蛋白 基因启动子部分序列的进化分析[J]. 遗传, 2004, 26(3): 0-329
作者姓名:王宏林  喻德跃  王永军  陈受宜  盖钧镒WANG Hong-Lin  YU De-Yue  WANG Yong-Jun  CHEN Shou-Yi  GAI Jun-Yi
作者单位:中国科学院遗传与发育生物学研究所植物细胞于染色体工程国家重点实验室,北京100101 The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
摘    要:通过PCR克隆的方法,获得了分别来自二倍体长穗偃麦草的E基因组和四倍体长穗偃麦草的E1基因组的4个高分子量麦谷蛋白亚基(HMW-GS)基因启动子的部分序列。序列分析表明,它们之间的同源性较高,两个x型亚基启动子序列之间只有1个碱基的差异,而两个y型亚基启动子序列完全相同, x和y型亚基启动子序列之间的长度和部分碱基位点都有差异。推测四倍体长穗偃麦草中的E1基因组可能起源于二倍体的E基因组。与来自小麦族的A、B、D和G基因组部分亚基基因的启动子序列比较表明,小麦族的这一区域在进化上是相当保守的,不同基因组来源的序列同源性都在90%以上。经过对这些序列的聚类分析,表明长穗偃麦草的y型HMW-GS基因与其他亚基基因的进化关系较远,而x型亚基基因与一个来自小麦1B染色体的亚基基因关系最近。Abstract: The partial promoter regions of HMW glutenin subunit genes were cloned form the genomes E (in diploid Agropyron elongatum) and E1 (in tetraploid Agropyron elongatum) by PCR approach. There was only one nucleotide acid difference in the promoter sequences of x-type subunits between the two genomes; moreover, the promoter sequences of the two y-type subunits were completely identical. Although these promoter regions were very similar to each other, differences still existed in sequence size and the kind of nucleotide acid between the x-type and y-type subunits. It was speculated that the E1 genome in tetraploid Agropyron elongatum was probably originated from E genome in diploid species. The comparisons of these subunits with some of those from A, B, D and G genome of Triticeae demonstrated that the sequences of their partial promoter regions were conserved and shared a high homology more than 90%. The phylogenetic analysis based on the sequences in this region indicated that the y-type HMW glutenin subunits of Agropyron elongatum species were different from other subunits, whereas the x-type subunits of them were most closely related to that from the B genome.

关 键 词:长穗偃麦草  高分子量麦谷蛋白基因  启动子  Agropyron elongatum  进化 Key words  

Mapping QTLs of Soybean Root Weight With RIL Population NJRIKY
WANG Hong-Lin,YU De-Yue,WANG Yong-Jun,CHEN Shou-Yi,GAI Jun-Yi. Mapping QTLs of Soybean Root Weight With RIL Population NJRIKY[J]. Hereditas, 2004, 26(3): 0-329
Authors:WANG Hong-Lin  YU De-Yue  WANG Yong-Jun  CHEN Shou-Yi  GAI Jun-Yi
Abstract:
Keywords:
点击此处可从《遗传》浏览原始摘要信息
点击此处可从《遗传》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号