Possible role of prostaglandin F in blastocyst implantation |
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| Authors: | Mitsunori Kasamo Mutsuo Ishikawa Kohki Yamashita Kazuo Sengoku Tetsuya Shimizu |
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| Affiliation: | 1. Mech-Sense, Department of Gastroenterology and Hepatology, Aalborg University Hospital, Mølleparkvej 4, Aalborg 9000, Denmark;2. Department of Clinical Medicine, Aalborg University, Søndre Skovvej 15, Aalborg 9000, Denmark;3. Department of Psychology and Behavioural Sciences, Aarhus University, Bartolins Allé 11, Aarhus 8000, Denmark;4. Department of Drug Design and Pharmacology, University of Copenhagen, Jagtvej 160/162, Copenhagen 2100, Denmark |
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| Abstract: | The synthesis and release of Prostaglandin F (PGF) by the rabbit blastocyst and endometrium were investigated on Day 6 and 7, using radioimmunoassay, autoradiography and conversion experiments. The following results were obtained: - 1.1) The content of PGF in the blastocyst increased significantly (P < 0.01) form Day 6 and 7.
- 2.2) The content of PGF in the endometrium was significantly higher (P < 0.05) on Day 7 implantation sites compared to the other areas.
- 3.3) The in vitro synthesis and release of PGF by Day 6 balstocysts sharply increased after one and two hours of culture, respectively. Thereafter both values declined with time.
- 4.4) The in vitro synthesis and release of PGF by Day 6 endometria increased continuously with time.
- 5.5) 14C-arachidonic acid (14C-AA) was incorporated into Day 6 blastocysts in vitro and converted to PGF2α. These results suggest that both the endometrium and the blastocyst are the sources of the PGs involved in implantation, and that PGF derived from the blastocysts may act as the trigger of implantation.
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