|
|||||
|
|
Erythropoietic progenitor cells from premature neonates studied using a validated serum-deprived medium |
|
| Authors: | Nigel Barry Westwood Anthony John Barrett Emmerson |
| Affiliation: | (1) Division of Haematology, United medical School of Guy's St Thomas' Hospital, University of London, St Thomas' Hospital Campus, London, U.K.;(2) Dental School of Guy's St Thomas' Hospital, University of London, St Thomas' Hospital Campus, London, U.K.;(3) Division of Paediatrics, United Medical School of Guy's St Thomas' Hospital, University of London, St Thomas' Hospital Campus, London, U.K.;(4) Dental School of Guy's St Thomas' Hospital, University of London, St Thomas' Hospital Campus, London, U.K.;(5) Department of Haematology, St Thomas' Hospital, Lambeth Palace Road, SE1 7EH London, U.K. |
| Abstract: | We have examined a serum-deprived culture system in order to verify that it is suitable for the study of burst forming unit erythroid (BFU-E) progenitor cells from premature neonates. Optimum growth of BFU-E from premature neonates was observed with each media constituent using the same concentration as that previously described for adult subjects. Growth of immature BFU-E from premature neonates were highly dependant upon a source of Burst Promoting Activity and mature BFU-E derived colonies emerged at day 12 compared to day 14 in adults. Our preliminary results with the validated medium suggest that premature infants have increased peripheral blood concentrations of BFU-E compared to healthy adult controls.Abbreviations Ad Adherent cells - BPA Burst promoting activity - BFU-E Burst forming unit erythroid - Epo Erythropoietin - IL3 Interleukin-3 - LDC Low density (<1.077 g ml1) peripheral blood mononuclear cells |
| Keywords: | BFU-E clonal growth erythropoietic bursts serum-free medium tissue culture technique |
| 本文献已被 SpringerLink 等数据库收录！ | |