Developmental Regulation of Neuregulin1 Isoforms and erbB Receptor Expression in Intact Rat Dorsal Root Ganglia |
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Authors: | Sara Reinhard Eric Vela Nicole Bombara George H DeVries Timothy D Raabe |
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Institution: | (1) Department of Biological Sciences, St. Mary’s University, One Camino Santa Maria, San Antonio, TX 78228, USA;(2) Research Service, Hines VA Hospital, Fifth Ave./Roosevelt Rd., Hines, IL 60141, USA;(3) Department of Anatomy and Cell Biology, University of Illinois-Chicago, 600 S. Wood St., Chicago, IL 60612, USA |
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Abstract: | Neuregulins (NRGs) are a family of growth factors which bind to the erbB family of tyrosine kinase receptors. The exact nature
and interaction of specific NRG isoforms and erbB receptors that occur during the development of the nervous system have not
been reported. In order to better understand the role that different NRG isoforms and erbB receptors play in the differentiation,
proliferation, and survival of neurons and glial cells, we isolated protein and mRNA from dorsal root ganglia of rat pups
between embryonic day (E) 13 and postnatal day (P) 15. The relative expression levels of the NRGs and erbB receptors for the
different time points were compared using both Western and RT-PCR analyses. NRG1-type1α protein levels were highest at E-13
and then decreased by approximately 40% and remained constant through P-15. In contrast, mRNA levels for NRG1-type1α remained
constant from E-15 to P-15. The protein levels for NRG1-type 1β were similar to NRG1-type1α at E-13 with an approximate 40%
increase in the levels at E-15 and E-17 followed by a decrease to E-13 levels for the remainder of the developmental time
periods. The mRNA levels for NRG1-type1β remained constant from E-15 to P-15. The protein and mRNA expression patterns for
each erbB receptor were distinctive. The protein levels for erbB-2 were highest at E-19 while erbB-3 levels were highest at
E-17 and E-18. ErbB-4 protein levels were highest at E-13 and decreased through P-15. The developmental pattern for erbB-2
and erbB-4 mRNA levels had no relation to that of the corresponding protein levels while the mRNA levels for erbB-3 were highest
at E-17 and E-18 similar to the pattern observed for the erbB-3 protein levels. We concluded that both NRG and erbB expression
in dorsal root ganglia are mostly translationally controlled and that NRG1 isoforms and their erbB receptors are not coordinately
regulated.
Special issue article in honor of Dr. George DeVries. |
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Keywords: | Neuregulin1-type 1α Neuregulin1-type 1β erbB-2 erbB-3 erbB-4 Dorsal root ganglia |
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