FTIR spectroscopic discrimination of Saccharomyces cerevisiae and Saccharomyces bayanus strains |
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Authors: | Adt Isabelle Kohler Achim Gognies Sabine Budin Julien Sandt Christophe Belarbi Abdelkader Manfait Michel Sockalingum Ganesh D |
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Institution: | MéDIAN, Université de Reims Champagne-Ardenne, MEDyC CNRS UMR 6237, UFR Pharmacie, IFR53, 51 rue Cognacq-jay, 51096 REIMS CEDEX, France. isabelle.adt@iut.univ-lyon1.fr |
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Abstract: | In this study, we tested the potential of Fourier-transform infrared absorption spectroscopy to screen, on the one hand, Saccharomyces cerevisiae and non-S. cerevisiae strains and, on the other hand, to discriminate between S. cerevisiae and Saccharomyces bayanus strains. Principal components analysis (PCA), used to compare 20 S. cerevisiae and 21 non-Saccharomyces strains, showed only 2 misclassifications. The PCA model was then used to classify spectra from 14 Samos strains. All 14 Samos strains clustered together with the S. cerevisiae group. This result was confirmed by a routinely used electrophoretic pattern obtained by pulsed-field gel electrophoresis. The method was then tested to compare S. cerevisiae and S. bayanus strains. Our results indicate that identification at the strain level is possible. This first result shows that yeast classification and S. bayanus identification can be feasible in a single measurement. |
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