Mutation of the RGD sequence does not affect plasma membrane association and growth inhibitory effects of elevated IGFBP-2 in vivo |
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Authors: | Hoeflich A Reisinger R Vargas G A Elmlinger M W Schuett B Jehle P M Renner-Müller I Lahm H Russo V C Wolf E |
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Institution: | Institut de Botanique, Laboratoire de Physiologie Végétale, University of Neuchatel, CH-2007 Neuchatel, Switzerland. |
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Abstract: | Vacuolar sequestration or cellular extrusion of glutathione-conjugated xenobiotics and catabolites by ATP-binding cassette (ABC) transporters is an important detoxification mechanism operating in many species. In this study, we show that the yeast ABC transporter Bpt1p, a paralogue of Ycf1p, acts as an ATP-dependent vacuolar pump for glutathione conjugates. Bpt1p, which is inhibited by vanadate and glibenclamide, accounts for one third of the total vacuolar transport of glutathione conjugates. Furthermore, immunoblot analyses show that Bpt1p levels are strongly elevated in early stationary phase, consistent with a function of Bpt1p in vacuolar detoxification. |
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Keywords: | Yeast Vacuole Glutathione ATP-binding cassette transporter Detoxification Stationary phase |
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