海金沙精细胞中生毛体发育及多层结构起源的超微结构研究

蕨类植物海金沙（LygodiumJaponicum（Thunb．）Sw．）的游动精子发育过程中，生毛体在精母细胞的细胞质中出现，它是直径为0．5－0．6μm的椭球体，其结构紧密，由辐射排列的具轮辐结构的管状亚单位和无定形基质组成。大量微管从生毛体伸向细胞质。随着精细胞的发育，生毛体结构变得松散，亚单位分化形成的中心粒彼此分开扩散到外围，中心为无定形物质。伴随着中心粒的分化，多层结构出现，一端与无定形基质相连。多层结构由外侧的微管带及内侧的片层组成，形成后与一线粒体相连，移向靠近核的位置，并正对着核上出现凹点。研究发现在精原细胞后期出现一团絮状结构，为无定形基质，其中有深染色的小管状结构分布，同时可见微管从絮状结构边缘伸出，这一絮状结构可能与生毛体的产生有一定的关系。

An Ultrastructural Study of the Development of Blepharoplast and the Origin of Multilayered Structure in the Sperm of Lygodium japonicum

The blepharoplast, an elliptical, compact spherical body ranging 0. 5 ~ 0. 6 um in diameter,appears in the cytoplasm of the sperm mother cell during the sperm development of Lygodium japonicum (Thunb. ) Sw. It consists of cartwheel tubular subunits arranged in radiant pattern and amorphous material.Numerous microtubules extended from the blepharoplast into cytoplasm. In the process of sperm cell development, the blepharoplast became loose, and the centrioles originated from the subunits separated from each other and dispersed towards the surface, with the amorphous material in the center. Accompanying the differentiation of the centrioles, multilayered structure with the spline (i. e. microtubular band) and lamella strata came into existence, with one end attaching to the amorphous material in the center. Then the multilayered stmcture (MLs) associated with a mitochondrion and moved towards the nucleus, and a dentfacing the MLs is formed in the nucleus. A cotton-like structure consisting of amorphous material and dardyed small tubular structure appeard in late stage of spermatogenesis. Microtubules were seen protruding from the surface of the cotton-like structure. It is suggested that this structure might be relevant to the origin of the blepharoplast.