黄精凝集素PCL-2诱导人前列腺癌LNCap细胞凋亡及机制研究

研究黄精凝集素PCL-2对人前列腺癌LNCap细胞生物活性和可能的抗肿瘤机制。从黄精药材中提取分离得到黄精凝集素PCL-2,体外培养LNCap细胞,采用WST-1和集落形成实验评价PCL-2对LNCap细胞的增殖作用;2,7-二氯荧光黄双乙酸盐(DCFH-DA)荧光探针法检测LNCap细胞内ROS含量;qRT-PCR和Western blot法检测PCL-2对LNCap细胞中相关基因和蛋白表达的影响。研究结果显示PCL-2能显著抑制LNCap细胞的增殖,促进细胞中ROS生成;PCL-2通过上调LNCap细胞中Bax、Caspase-3及Caspase-9基因mRNA和蛋白表达并下调Bcl-2基因mRNA和蛋白表达水平诱导LNCap细胞凋亡。本研究结果表明PCL-2诱导LNCap细胞凋亡活性作用显著,可作为抑制前列腺癌的潜在药物。

Lectin PCL-2 of Polygonatum cyrtonema induces apoptosis of human prostate cancer LNCap cells and its mechanism

In this study, the biological activity of lectin PCL-2 of Polygonatum cyrtonema on human prostate cancer LNCap cells and its possible anti-tumor mechanism were explored.PCL-2 was extracted and isolated from P.cyrtonema fresh medicinal materials, and LNCap cells were cultured in vitro.WST-1 and colony formation experiments were used to detect the proliferation of LNCap cells.The level of ROS in LNCap cells were determined by 2,7-dichlorofluorescein yellow diacetate(DCFH-DA) fluorescence probe method.The expression levels of related genes and proteins in LNCap cells was measured by qRT-PCR and Western blot.Activity study results show that PCL-2 can significantly inhibit the proliferation of LNCap cells, promote the generation of ROS in cells.Mechanism research results show that PCL-2 induces LNCap cells apoptosis by up-regulating the mRNA expression levels of Bax, Caspase-3 and Caspase-9 genes and proteins and down-regulating the mRNA expression levels of Bcl-2 genes and proteins.The results of this study show that PCL-2 has a significant effect in inducing apoptosis of LNCap cells, and it can be used as a potential drug for inhibiting prostate cancer.