川芎内生菌Pseudeurotium ovale代谢产物研究

通过大孔树脂柱层析、凝胶柱层析和高效液相色谱等方法对川芎中分离得到的内生菌Pseudeurotium ovale的代谢物进行分离纯化,从中分离得到5个化合物,并通过核磁共振和质谱等波谱学技术手段确认所有化合物的结构,其中包含一个新的化合物4-methoxy-3-methyl-6-(1 E,3 E)-1,3-pentadien-1-yl-2 H-pyran-2-one(1)和四个首次从中分离得到的已知化合物:(2 E,4 E)-1-(2,6-dihydroxy-3,5-dimethylphenyl)-2,4-hexadien-1-one(2)、2-deoxy-sohirnone C(3)、trichodimerol(4)、3,7-dihydroxy-1,9-dimethyl-dibenzofuran(5)。体外抗炎活性测试表明化合物1可抑制经脂多糖(LPS)处理的RAW 264.7细胞中NO、IL-6和TNF-α等炎症因子的分泌。

Study on metabolites of Pseudeurotium ovale isolated from Ligusticum chuanxiong

Five compounds, including a new one(1),were isolated from the broth of Pseudeurotium ovale,an endophytic fungus of Ligusticum chuanxiong by some column chromatographic such as macroporous resin column chromatography, gel column chromatography and HPLC.Their structures were elucidated as 4-methoxy-3-methyl-6-(1E,3E)-1,3-pentadien-1-yl-2H-pyran-2-one(1),(2E,4E)-1-(2,6-dihydroxy-3,5-dimethylphenyl)-2,4-hexadien-1-one(2),2-deoxy-sohirnone C(3),trichodimerol(4),3,7-dihydroxy-1,9-dimethyl-dibenzofuran(5) by the interpretation of their spectroscopical evidences from 1 D and 2 D NMR as well as HR-ESI-MS.Compound 1 exhibited anti-inflammatory properties in vitro by attenuating the production of inflammatory mediators, such as nitric oxide(NO) as well as tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6).