水分胁迫对抗旱性不同的荔枝叶片细胞壁H+-ATPase活性的影响

以抗旱性较强的荔枝东刘1号和抗旱性较弱的陈紫1－2年分盆栽实生幼苗为试验材料。研究了水分胁迫对荔枝（Litchi chinensis Sonn）叶片细胞胞质以及以离子键和共价键与细胞壁结合的H^ -ATPase活性的影响。结果表明：（1）叶片相对含水量随水分胁迫程度的增加而减少，抗旱性强的东刘1号下降的幅度小于抗旱性弱的陈紫。（2）H^ -ATPase在细胞中的分布是：细胞胞质H^ -ATPase占绝大多数，其次是共价键结合H^ -ATPase，离子键结合H^ -ATPase最少，品种间差异不明显。（3）在水分胁迫下，荔枝叶片H^ -ATPase活性（比活性）均上升，抗旱性强的品种上升的幅度均大于抗旱性弱的品种。

EFFECTS OF WATER STRESS ON CELL WALL H+-ATPase ACTIVITY IN LEAVES OF LITCHI CHINENSIS SONN. WITH DIFFERENT DROUGHT-RESISTANCES

One to 2-year-old seedlings of two lychee (Litchi chinensis Sonn.) cultivars (droughtsensitive Chenzi and drought-resistant Dongliu 1) were planted in pots by moisture stress and normal watering to determine the relative water content (RWC) and the H -ATPase activities in leaves. The effects of water stress on (specific) activity of cytoplasmic, ionically and covalently wall-bound H -ATPase in leaves were studied. The results showed that RWC in leaves decreased with increasing water stress, but such decrease was less in droughtresistant cultivar than in drought-sensitive one. The activities of cytoplasmic, covalently wall-bound and ionically wall-bound H -ATPase were 81.80% - 83.15%, 11.48% - 12.30% and 5.37%-5.90% of the total H -ATPase activity, respectively. No significant difference was observed between two cultivars. Under water stress, the (specific) activities of Cytoplasmic, ionically wall-bound and covalently wall-bound H -ATPase in leaves increased. The extent of increase was higher in drought-resistant cultivar than in drought-sensitive one.