Rapid micropropagation for Mucuna pruriens f. pruriens L. |
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Authors: | Sharmila Chattopadhyay S K Datta S B Mahato |
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Institution: | (1) Department of Botany, Visva Bharati University, 731235 Santiniketan, India;(2) Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, 700032 Calcutta, India |
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Abstract: | A rapid micropropagation system was developed for Mucuna pruriens f. pruriens using explants from 1-week-old aseptically grown seedlings. Multiple shoot regeneration occurred following an initial callus growth on Revised Tobacco (RT) medium supplemented with 2.7 M NAA and 9.8 M 2iP. Maximum number of shoot regeneration was achieved only from seedling explant 6 to 7 days old. More than 90% of the regenerated shoots could be rooted on half-strength liquid RT medium supplemented with 2.7 M NAA. Plantlets readily adopted to greenhouse conditions. This system provides a new tool for micropropagation of Mucuna pruriens f. pruriens, an important medicinal plant.Abbreviations BAP
6-benzylaminopurine
- Kn
kinetin
- 2iP
2-isopentenyladenine
- AdS
adenine sulphate
- NAA
alpha-naphthalene acetic acid
- IAA
indole-3-acetic acid
- IBA
indole-3-butyric acid
- MS
Murashige and Skoog (1962) medium
- RT
Revised Tobacco medium (Kaul and Staba, 1968) |
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Keywords: | |
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