Host-specific nodulation is encoded on a 14kb DNA fragment in Rhizobium trifolii |
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Authors: | Peter R. Schofield Robert W. Ridge Barry G. Rolfe John Shine John M. Watson |
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Affiliation: | (1) Centre for Recombinant DNA Research, Australian National University, G.P.O. Box 4, 2601 Canberra City, Australia;(2) Department of Genetics, Research School of Biological Sciences, Australian National University, G.P.O. Box 4, 2601 Canberra City, Australia |
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Abstract: | Summary The Rhizobium trifolii genes necessary for nodule induction and development have been isolated on a 14.0kb fragment of symbiotic (Sym) plasmid DNA. When cloned into a broad-host-range plasmid vector, these sequences confer a clover nodulation phenotype on a derivative of R. trifolii which has been cured of its endogenous Sym plasmid. Furthermore, these sequences encode both host specificity and nodulation functions since they confer the ability to recognize and nodulate clover plants on Agrobacterium and a fast-growing cowpea Rhizobium. This indicates that the bacterial genes essential for the initial, highly-specific interaction with plants are closely linked. |
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Keywords: | host specificity nodulation genes Rhizobium trifolii Sym plasmid symbiotic nitrogen fixation |
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