| 苦荞叶片超氧化物歧化酶的纯化及性质研究 |
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| 引用本文： | 王转花,张政,林汝法,周明德. 苦荞叶片超氧化物歧化酶的纯化及性质研究[J]. 中国生物化学与分子生物学报, 1994, 10(5): 539-543 |
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| 作者姓名： | 王转花 张政 林汝法 周明德 |
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| 作者单位： | 山西大学生物系，山西省农业科学院小杂粮室 |
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| 基金项目： | 国际植物遗传资源委员会（IBPGR）资助课题,山西省自然科学基金 |
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| 摘 要： | 从荞麦生化遗传以及器官衰老机理的研究目的出发，以苦荞叶片为材料，制备出活力较高的铜锌趋氧化物歧化酶。对其理化性质分析表明：该酶在２５９ｎｍ处有一特征吸收峰，分子量约为３１ｋＤ，含有３０８个氨基酸残基，同工酶电泳结果显示三条活性带。
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| 关 键 词： | 苦荞叶片；超氧化物歧化酶；纯化；性质 |
| 收稿时间： | 1994-10-20 |
Studies on the Purification and Characterization of Superoxide Dismutase from Tartary Buckwheat Leaves |
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| Wang Zhuan-hua,Zhang Zheng. Studies on the Purification and Characterization of Superoxide Dismutase from Tartary Buckwheat Leaves[J]. Chinese Journal of Biochemistry and Molecular Biology, 1994, 10(5): 539-543 |
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| Authors: | Wang Zhuan-hua Zhang Zheng |
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| Affiliation: | (Departmeut of Biology, Shanxi University,Taiyuan 030006)Lin, Ru-fa (Small Grain Programme, Shanxi Academy of Agricultural Sciences, Taiyuan)Zhou, Ming-de (IBPGR Office for East Asia c/o CAAS, Beijing |
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| Abstract: | Copper and zinc-containing superoxide dismutase with high specific activity was prepared from fresh tartary buckwheat leaves. The molecular weight of the purified enzyme is about 31kD, a maximum UV-absorption of the enzyme is found at 259nm, and the analysis of amino acid composition showed that the enzyme contains 308 amino acid residues.The purified enzyme showed three isozyme bands by PAGE. |
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| Keywords: | Tartary buckwheat leaves Superoxide dismutase Purification Characterization |
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