A simple reproducible method for prometaphase chromosome analysis |
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| Authors: | Joseph Rybak Avirachan Tharapel Sheldon Robinett Mary Garcia Carl Mankinen Mahlon Freeman |
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| Affiliation: | (1) Genetics Screening and Counseling Service, Texas Department of Mental Health and Mental Retardation, 76201 Denton, Texas, USA;(2) Present address: Department of Pathology, UTHSC-Dallas, 75235 Dallas, Texas;(3) Present address: Department of Pediatric, Children's Hospital of Buffalo, SUNYAB, 219 Bryant Street, 14222 Buffalo, New York |
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| Abstract: | Summary A method is described for the analysis of chromosomes in prophase and early metaphase. It involves culturing the lymphocytes in medium RPMI-1640, supplemented with 10% autologous plasma instead of fetal bovine serum. Living cells are treated with actinomycin D and colcemid for 1 h prior to harvest and harvested early at 65 h of incubation, using a hypotonic solution formulated by Ohnuki (1968). The method has been tested on several hundred clinical samples on a routine basis. On average, 30% of the dividing cells were in prometaphase. |
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