Siderophore mediated plant growth promotion at low temperature by mutant of fluorescent pseudomonad⋆ |
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Authors: | Vandana Katiyar Reeta Goel |
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Affiliation: | (1) Department of Microbiology, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar, 263145 Uttaranchal, India |
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Abstract: | A cold resistant mutant of Pseudomonas fluorescens ATCC 13525 was developed, which could grow equally well at 25 and 10 °C and its effect on plant growth promotion under in vitro and in situ conditions was observed. Siderophore estimation revealed it to be a siderophore-overproducing mutant (17-fold increase) when compared to its wild type counterpart. A gnotobiotic root elongation assay indicated that the mutant (CRPF9) promoted growth more than its wild type both at 25 and 10 °C, indicating its effectiveness at low temperature. Further, root colonization studies showed that CRPF9 was an efficient rhizosphere colonizer, inducing a significant increase in root (35%) and shoot length (28%) of mung bean plants in unsterilized soil system. The persistence and stability of the mutant was evident in rhizospheric soil. A sand culture experiment showed that ferric citrate was better than Fe(OH)3 as an iron source for plant growth, but in the presence of CRPF9 both salts were comparable. This study demonstrates the potential of chemical mutagenesis for improving the plant growth promoting properties of a P. fluorescens strain and its stimulating impact on plant growth promotion at low temperature both under in vitro and in situ conditions. |
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Keywords: | Cold resistant mutant Fluorescent pseudomonads Low temperature Plant growth promotion Siderophore |
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