pUCPCR1 |
| |
Authors: | Erik de Vries |
| |
Institution: | (1) Department of Parasitology and Tropical Veterinary Medicine, Utrecht University, PO Box 80165, 3508 TD Utrecht, The Netherlands |
| |
Abstract: | The mutiple cloning site of pUC19 was replaced by a multiple cloning site possessing a double Xcm1 restriction site. Digestion
withXcmI gives a linear vector with a single 3′-overhanging T-residue at both ends. This provides the easiest way of creating a vector
in which PCR fragments produced byTaq polymerase can be directly cloned without further modifications. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|