The human dynamin-related protein OPA1 is anchored to the mitochondrial inner membrane facing the inter-membrane space |
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| Authors: | Olichon Aurélien Emorine Laurent J Descoins Eric Pelloquin Laetitia Brichese Laetitia Gas Nicole Guillou Emmanuelle Delettre Cécile Valette Annie Hamel Christian P Ducommun Bernard Lenaers Guy Belenguer Pascale |
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| Affiliation: | Biochemistry, Endocrinology and Metabolism Unit, Institute of Child Health (University College London), 30 Guilford Street, WC1N 1EH, London, UK. |
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| Abstract: | We have examined the effect of transforming growth factor β1 (TGF-β1) and overexpression of the Smad4 gene on the phenotype of Car C, a ras mutated highly malignant spindle carcinoma cell line. TGF-β1-treated Car C cells overexpressing Smad4 spread with a flattened morphology with membrane ruffles abundant in vinculin and show a reduction in their invasive abilities. TGF-β1 treatment and overexpression of Smad4 also enhanced the production of PAI-1 measured by the activation of the p3TP-lux reporter gene containing a PAI-1-related promoter. This activation was abolished with a dominant-negative Smad4 construct. These results lead us to conclude that both TGF-β1 and Smad4 overexpression reduce the invasive potential of Car C cells, probably via the Smad pathway. |
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| Keywords: | TGF-B1 Smad4 Spindle Cat C |
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