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On the cellular localization of phosphoenolpyruvate carboxylase in Sorghum leaves |
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| Authors: | Catherine Perrot Jean Vidal Arlette Burlet Pierre Gadal |
| Affiliation: | (1) Laboratoire de Biologie Végétale, Université de Nancy I, ERA au CNRS No 799, C/O No 140, F-54037 Nancy Cedex, France;(2) Present address: Laboratoire d'Histologie A, Facultés de Médecine, B.P. 184, F-54500 Vandoeuvre les Nancy, France |
| Abstract: | The localization of phosphoenol pyruvate carboxylase (EC 4.1.1.3.1.) in the leaf cells of Sorghum vulgare was investigated by using three techniques: the conventional aqueous and non aqueous methods gave conflicting results; the immunocytochemical techniques clearly showed that the enzyme is predominantly located in the cytoplasm of mesophyll cells.Abbreviations PEP phosphoenol pyruvate - PAG polyacrylamide gel - NADP MDH NADP malate dehydrogenase - FITC fluorescein isothiocyanate - SAB serum albumine bovine - DTT dithiothreitol - MDH malate dehydrogenase - ME malic enzyme - PBS phosphate buffer saline - PAP peroxidase anti-peroxidase |
| Keywords: | Immunocytochemistry (PEP carboxylase) PEP carboxylase Sorghum |
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