| 表面活性剂对海洋微生物溶菌酶活性的影响 |
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| 引用本文： | 张琇,任贤,王薇,刘雅琴. 表面活性剂对海洋微生物溶菌酶活性的影响[J]. 工业微生物, 2009, 39(6): 34-37. DOI: 10.3969/j.issn.1001-6678.2009.06.008 |
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| 作者姓名： | 张琇 任贤 王薇 刘雅琴 |
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| 作者单位： | 北方民族大学,生物科学与工程学院,银川,750021;国家民委发酵酿造工程生物技术重点实验室,银川,750021 |
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| 基金项目： | 国家民委教学改革与质量建设研究项目资助;北方民族大学引进人才项目资助 |
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| 摘 要： | 以海洋微生物溶菌酶（ⅧL）为研究对象，分别检验几种表面剂对MBL活性的影响，着重研究烷基多苷（APG）对其活性的影响。结果表明，APG与阳离子烷基多苷（矾PG）分别提高MBL相对酶活性为21％，15％，SDS降低该酶活性约为15％，Tween20和Tween80对MBL活性的影响不明显。MBL含量大于5．0mg／mL时，对大肠杆菌、金黄色葡萄、白色念珠球菌有抑菌作用。0．5％～1．5％的APG无明显抑菌作用。将5．0mg／mMBL与1．0mg／mLAPG复配后（简称CEP），发现APG能明显增强MBL抑菌作用，CEP具有较好地杀菌作用；CEP在54℃培养箱中放置14d后，其杀菌率保持不变，说明CEP的杀菌性能的稳定性良好。
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| 关 键 词： | 海洋微生物溶菌酶 表面活性剂 杀菌作用 |
Effects of several surfactants on the activities of marine microorganism lysozyme |
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| ZHANG Xiu,REN Xian,WANG Wei,LIU Ya-qing. Effects of several surfactants on the activities of marine microorganism lysozyme[J]. Industrial Microbiology, 2009, 39(6): 34-37. DOI: 10.3969/j.issn.1001-6678.2009.06.008 |
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| Authors: | ZHANG Xiu REN Xian WANG Wei LIU Ya-qing |
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| Affiliation: | ZHANG Xiu, REN Xian, WANG Wei, LIU Ya-qing(College of Biological Sciences and Engineering, North University for Ethnics, Yinchuan 750021, P.R. China;Key Laboratory of Ferrmentation, Brewing engineering and Biotechnology, State Nationalities Affairs Commission, Yinchuan 750021, P.R.China) |
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| Abstract: | The effects of several surfactants on the activities of marine microorganism lysozyme were investigated,especially the effects of alkyl poly glycosides(APG)on the enzyme.The results indicated that both APG and cationic alkyl poly glycosides(cAPG) improved the relative activity of the enzyme with 21% and 15% of its maximum activity,respectively;SDS lost the activity of the enzyme relative activity about 15% of its maximum activity.Tween 20 and Tween 80 had no effects on the enzyme relative activity.The results also showed that the solution with concentration of more than 5.0 mg/mL MBL had effective antimicrobial activity to Escherichia coli,Staphylococcus aureus or Candida albicans.The solution containing 0.25 mg/mL～1.0 mg/mL APG was almost ineffective on antimicrobial activity.5.0 mg/mL MBL plus with available 1.0 mg/mL APG (compound enzyme preparation,CEP) had better bacteriostastic activity.When CEP was placed in culture incubator for two weeks at 54 ℃,the killing rates of CEP kept stable.It was concluded that CEP killed bacteria effectually. |
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| Keywords: | marine microorganism lysozyme surfactants antimicrobial activity |
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