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Evidence that S100 proteins regulate microtubule assembly and stability in rat brain extracts
Authors:J Hesketh  J Baudier
Affiliation:1. Rowett Research Institute, Bucksburn, Aberdeen AB2 9SB, U.K.;2. Laboratoire de Physique, UA CNRS 491, UER des Sciences Pharmacetiques, B.P. No 10, 67048 Strasbourgh, France;1. Key Laboratory of Immune Response and Immunotherapy, Center for Advanced Interdisciplinary Science and Biomedicine of IHM, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230027, Anhui, China;2. Department of Geriatrics, Gerontology Institute of Anhui Province, The First Affiliated Hospital of University of Science and Technology of China, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, China;1. Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy;2. School of Pharmacy, University of Camerino, Camerino, Italy;1. Department of Hematology and Key Laboratory of Non-resolving Inflammation and Cancer of Hunan Province, The 3rd Xiangya Hospital, Central South University, Changsha 410000, P.R. China;2. Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA;3. Department of Physiology, School of Basic Medical Science, Central South University, Changsha, Hunan Province 410000, P.R. China;4. Key Laboratory of Medical Genetics, School of Biological Science and Technology, Central South University, Changsha, Hunan Province 410000, P.R. China;5. Key Laboratory of Sepsis Translational Medicine of Hunan, Central South University, Changsha, Hunan Province 410000, P.R. China;6. College of Life Science, Hunan Normal University, Changsha 410081, P.R. China;7. Shanghai Institute of Materia Medica, Chinese Academy of Science, 501 Hai-ke Rd, Shanghai 201203, P.R. China;8. College of Chemistry, Nankai University, Tianjin 300073, P.R. China;9. Center for Biologic Imaging, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA;10. Laboratory of Biomedical Science, The Feinstein Institute for Medical Research, Northwell Health, 350 Community Drive, Manhasset, NY 11030, USA;11. Department of Emergency Medicine, North Shore University Hospital, Northwell Health, 350 Community Drive, Manhasset, NY 11030, USA;1. Department of Pathology and Comprehensive Cancer Center, University of Michigan, Ann Arbor, MI 48109, USA;2. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
Abstract:Microtubule re-assembly in rat brain extracts was inhibited by antibodies to S100 proteins. Anti-S100 antibodies caused an increase in the cold-stability of microtubules and this effect was abolished by the presence of short lengths of microtubules formed under control conditions. Anti-S100 antibodies had no effect on the stimulation of assembly or the increase in microtubule stability caused by low zinc concentrations. Addition of exogenous S100a and S100b to brain extracts had different effects on assembly; S100a caused an inhibition of assembly while S100b stimulated the early phase of assembly. The data suggest that endogenous S100b is involved in the regulation of microtubule assembly in brain extracts.
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