Estimation of luminal mucin content in rats by measurement of O-linked oligosaccharide chains and direct ELISA |
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| Authors: | Tanabe Hiroki Ito Hiroyuki Sugiyama Kimio Kiriyama Shuhachi Morita Tatsuya |
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| Institution: | Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Shizuoka, Japan. |
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| Abstract: | Changes in the small intestinal mucin contents in rats were evaluated by two methods, viz., a newly established ELISA and a method based on the measurement of O-linked oligosaccharide chains (OSC) as a mucin marker. Significant correlation was observed between the values of ELISA-derived mucins and OSC. The results confirm the usefulness of measurement of OSC as an alternative method for mucin determination. |
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