Construction of EGFP-tagged rBCG of E.tenella and distribution in chickens |
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Authors: | QiuYue Wang JianHua Li XiChen Zhang ChengWu Liu LiLi Cao KeYan Ren PengTao Gong YaNan Cai |
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Institution: | (1) College of Animal Science and Veterinary Medicine, Jilin University, Changchun, 130062, China;(2) Shenyang Police Dog Training School, Shenyang, 110034, China;(3) Veterinary Science Institute of Jilin Province, Changchun, 130062, China |
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Abstract: | Chicken coccidiosis is a major parasitic disease with substantial economic burden to the poultry industry. Enhanced green
fluorescent protein (EGFP) tagged recombinant Bacille Calmette-Guerin (rBCG), as a fusion protein with coccidian rhomboid
antigen was constructed to track rBCG in vivo in chickens in this study. Immunization of chickens with one dose of rBCG pMV361-Rho/EGFP induced humoral immune response.
The colonization of rBCG in liver, spleen, lung, kidney and caecum was observed by laser confocal microscopy. Real-time quantitative
RT-PCR showed a rise expression level of rhomboid protein on the 7th day and a peak on the 14th day and disappearance on the
28th day after immunization. These results have significant implications for the development of rBCG vaccines against avian
coccidiosis.
Supported by Grants from the Foundation of the Jilin Provincial Science and Technology Department of China (Grant No. 20050211-2)
and the National Natural Science Foundation of China (Grant No.30170696, 30500370 and 30671580). |
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Keywords: | coccidiosis rBCG EGFP rhomboid laser confocal microscopy real-time RT-PCR |
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