Selective plating medium for enumeration and recovery of a hexachlorocyclohexane-degrading Pseudomonas Ptm+ strain from soil |
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| Authors: | K.A. Anu,Appaiah ,D. M. Shaw, N.G.K. Karanth, &dagger |
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| Affiliation: | Department of Applied Botany, University of Mysore, Manasa Gangothri, Mysore 570 006, India;*Infestation Control and Protectants Discipline, Central Food Technological Research Institute, Mysore 570 013, India |
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| Abstract: | A double selective plating medium has been developed to enumerate a Pseudomonas Ptm+ strain capable of assimilating hexachlorocyclohexane (HCH). The ability of the isolate to utilize HCH and/or streptomycin as sole sources of carbon was chosen to impart selectivity. In trials when serial 10-fold soil dilutions were spread on HCH plus streptomycin mineral agar and incubated, the normal microflora did not grow. When soil containing an HCH degrading Pseudomonas was cultured, only the target colonies developed. This double selective plating method is suitable for environmental monitoring of HCH scavengers as the medium is sensitive, specific and practical. |
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