Saponification of Methylated Mucosubstances at Low Temperatures |
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Authors: | Tapani E. Sorvari Peter J. Stoward |
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Affiliation: | a Department of Pathological Anatomy, University of Turku, Finlandb Department of Anatomy, University of Dundee, Dundee, Scotland |
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Abstract: | A fresh 1% solution of KOH in 70% ethanol in 2 hr at 2-4 C restores basophilia to methylated acid mucosubstances satisfactorily without detaching or damaging tissue sections. A 0.5% solution of Ba(OH)2 under the same conditions gives results nearly as good, but NaOH and KMnO4 are unsatisfactory. |
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