| 二氢叶酸还原酶基因在斑马鱼神经系统发育过程中的作用 |
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| 引用本文： | 孙淑娜,桂永浩,蒋璆,钱林溪,宋后燕.二氢叶酸还原酶基因在斑马鱼神经系统发育过程中的作用[J].中国实验动物学报,2010,18(2):127-130,I0006,I0007. |
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| 作者姓名： | 孙淑娜 桂永浩 蒋璆 钱林溪 宋后燕 |
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| 作者单位： | 1. 复旦大学附属儿科医院,上海,201102
2. 复旦大学分子医学教育部重点实验室,上海,200032 |
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| 基金项目： | 国家教育部博士点基金资助 |
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| 摘 要： | 目的观察二氢叶酸还原酶基因(DHFR)功能阻抑斑马鱼胚胎的颅脑部发育情况,初步探讨二氢叶酸还原酶基因在斑马鱼神经系统发育过程中的作用。方法采用显微注射吗啡啉修饰的反义核苷酸(MO)的方法进行DHFR表达阻抑。胚胎发育至受精后48hpf观察胚胎的颅部发育情况,在60hpf时经石蜡切片进一步观察胚胎的脑发育状况。利用胚胎整体原位杂交的方法检测影响神经系统发育的关键因子ngn1和huc的表达情况。结果显微注射MO可成功的进行DHFR表达阻抑。DHFR表达阻抑组胚胎存在颅脑部发育明显异常和ngn1、huc的表达强度明显减弱,且与显微注射的MO剂量呈正相关。结论DHFR在斑马鱼颅脑发育中有重要作用;其功能阻抑可导致胚胎颅脑部发育异常,其机理与ngn1和huc的的表达减弱有关。
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| 关 键 词： | 二氢叶酸还原酶基因 神经系统 斑马鱼 |
Effects of Dihydrofolate Reductase Gene on the Development of Nervous System in Zebrafish |
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| SUN Shu-na,GUI Yong-hao,JIANG Qiu,QIAN Lin-xi,SONG Hou-yan.Effects of Dihydrofolate Reductase Gene on the Development of Nervous System in Zebrafish[J].Acta Laboratorium Animalis Scientia Sinica,2010,18(2):127-130,I0006,I0007. |
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| Authors: | SUN Shu-na GUI Yong-hao JIANG Qiu QIAN Lin-xi SONG Hou-yan |
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| Institution: | SUN Shu-na1,GUI Yong-hao1,JIANG Qiu2,QIAN Lin-xi2,SONG Hou-yan2(1.Children\'s Hospital,Fudan University,Shanghai 200032,China,2.Department of Molecular Genetics,Shanghai Medical College , Key Laboratory of Molecular Medicine,Ministry of Education,Shanghai 200032) |
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| Abstract: | Objective To observe the development of cranium and brain in dihydrofolate reductase gene-inhibited zebrafish embryos and to investigate the mechanism of action of dihydrofolate reductase gene(DHFR) in this process.Methods DHFR was inhibited by microinjecting morpholino oligonucleotides(MO).The development of heads at 48 hours post fertilization(48 hpf) was observed and the development of the brain at 60 hpf was examined by histopathology.Whole-mount in situ hybridization was performed to detect the express... |
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| Keywords: | dihydrofolate reductase gene nervous system zebrafish |
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