Overexpression of yeast <Emphasis Type="Italic">S</Emphasis>-adenosylmethionine synthetase <Emphasis Type="Italic">metK</Emphasis> in <Emphasis Type="Italic">Streptomyces actuosus</Emphasis> leads to increased production of nosiheptide |
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Authors: | Xincheng Zhang Meiqing Fen Xunlong Shi Linquan Bai Pei Zhou |
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Institution: | (1) School of Pharmacy, Fudan University, Yi Xue Yuan Road 138, Shanghai, 200032, China;(2) Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai, 200030, China |
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Abstract: | S-Adenosylmethionine (SAM) is synthesized via the metabolic reaction involving adenosine triphosphate and l-methionine that is catalyzed by the enzyme S-adenosyl-l-methionine synthetase (SAM-s) and encoded by the gene metK. In the present study, metK with the absence of introns from Saccharomyces cerevisiae was introduced into Streptomyces actuosus, a nosiheptide (Nsh) producer. Intracellular SAM levels were determined by high-pressure liquid chromatography. Through optimizing
the nutrient content of the medium, it was shown that increased SAM production induced by the overexpression of SAM-s leads
to an increase in the intracellular cysteine pool and overproduction of Nsh in S. actuosus. This investigation shows that increased SAM promotes the elevated production of the non-ribosomal thiopeptide Nsh in Streptomyces sp. |
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Keywords: | Streptomyces actuosus S-Adenosylmethionine metK Cysteine Nosiheptide |
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