Simultaneous determination of tryptophan and its metabolites in mouse brain by high-performance liquid chromatography with fluorometric detection |
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| Authors: | J Yamada Y Sugimoto K Horisaka |
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| Institution: | Department of Pharmacology, Kobe Women s College of Pharmacy, Motoyamakita-Machi, Higashinada-Ku, Kobe 658, Japan |
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| Abstract: | A new method for the determination of tryptophan and its metabolites in a single mouse brain using high-performance liquid chromatography (HPLC) with fluorometric detection is described. Tryptophan, serotonin, 5-hydroxyindoleacetic acid, indoleacetic acid, and tryptophol were clearly separated by a C8 reverse-phase column. Tissue preparation is performed only to centrifuge homogenates of brain prior to the injection to HPLC. The sensitivity is in the range from 10 to 15 pg. |
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| Keywords: | 5-HT serotonin 5-HIAA 5-hydroxyindoleacetic acid IAA indoleacetic acid CSF cerebrospinal fluid TOL tryptophol |
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