Determination and optimization of a strong promoter element from <Emphasis Type="Italic">Bacillus amyloliquefaciens</Emphasis> by using a promoter probe vector期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Determination and optimization of a strong promoter element from <Emphasis Type="Italic">Bacillus amyloliquefaciens</Emphasis> by using a promoter probe vector

Authors:	Yuling Liao Bin Wang Yanrui Ye Li Pan

Institution:	1.School of Bioscience and Bioengineering,South China University of Technology,Guangzhou,People’s Republic of China;2.Star Lake Bioscience Co., Inc,Zhaoqing,People’s Republic of China;3.Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering,Guangzhou,People’s Republic of China

Abstract:	Objective To construct a promoter probe vector, pBE-bgaB, to screen strong promoters from Bacillus amyloliquefaciens. Results 266 colonies containing active promoter elements from the genomic DNA of B. amyloliquefaciens were identified. Among these, promoter P41 exhibited the strongest β-Gal activity in Escherichia coli and B. amyloliquefaciens. Sequence analysis showed that promoter P41 contained P_ykuN, a ykuN gene encoding flavodoxin. Optimization of the ribosome-binding site from P41 to P₃₈₂ improved β-Gal activity by ~ 200%. Conclusion A new strong promoter for protein expression and genetic engineering of Bacillus species.

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