Isolation of monochromosomal hybrids for mouse Chromosomes 3, 6, 10, 12, 14, and 18 |
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| Authors: | Sabile Abdelmajid Poras Isabelle Cherif Dora Goodfellow Peter Avner Philip |
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| Institution: | (1) Unité de Génétique Moléculaire Murine, Institut Pasteur, 25 rue du Docteur Roux, 75015 Paris, France, FR;(2) Genethon, 1 rue de l'Internationale, 91000 Evry, France, FR;(3) Centre d'Etudes du Polymorphisme Humain (CEPH), 27 rue Juliette Dodu, 75010 Paris, France, FR;(4) Department of Genetics, Cambridge University, Tennis Court Road, Cambridge CB2 3EH, UK, GB |
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| Abstract: | Mouse/human somatic cell hybrids constitute a valuable resource for both genetic and physical mapping. In this report, we
describe the production and characterization of a series of six monochromosomal hybrids generated by fusion of murine microcells
with intact human recipient cells. The presence of each mouse chromosome was characterized by PCR analysis and the integrity
of the mouse chromosome retained in the hybrids confirmed by fluorescence in situ hybridization (FISH) analysis.
Received: 22 August 1996 / Accepted: 19 September 1996 |
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