A comparison of HPLC pigment analyses and biovolume estimates of phytoplankton groups in an oligotrophic lake |
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Authors: | Buchaca Teresa; Felip Marisol; Catalan Jordi |
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Institution: | 1 Departament DEcologia I Centre de Recerca DAlta Muntanya, Universitat de Barcelona, Avgda. Diagonal 645, E-08028 Barcelona, Spain and 2 Centre DEstudis AvanÇats de Blanes (CEAB-CSIC), Accés Cala St. Francesc 14, E-17300 Blanes, Spain |
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Abstract: | Assessment of the contribution of distinct algal groups to phytoplanktonbiomass in oligotrophic lakes by marker pigments is comparedwith assessment by cell-counting biovolume estimates. Seasonalsamples from an oligotrophic alpine lake (Redon, Pyrenees) mostlyincluded species of chrysophytes, dinoflagellates, cryptophytesand chlorophytes. The chlorophyl a (Chl a) corresponding toeach algal group was estimated using HPLC pigment analyses andthe CHEMTAX program. Chl a estimates and biovolume showed asignificant correlation for all the groups during the ice-freeseason except for chlorophytes. However, some of the samplesfrom the initial phase of the ice cover presented a clear departurefrom the relationship during the ice-free period in most groups.On the other hand, the ratios between a specific marker pigmentand the biovolume of the marked algal group were significantlyconstant within the photic zone (>1% surface irradiance)for most of the pigments and groups, including chlorophytes.Nevertheless, the ratios increased and showed a large variabilityfor samples below the photic depth or below the ice cover. Theviolaxanthin-chrysophyte biovolume ratio presented an opposedtendency to other pigment-biovolume ratios, which increasedin inverse proportion to the depth of the sample. The resultsare discussed in terms of methodological limitations, acclimationresponses and species composition. |
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