Substrate specificity of regiospecific desaturation of aliphatic compounds by a mutant Rhodococcus strain |
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Authors: | Koike K Takaiwa M Kimura Y Inoue S Ito S |
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Affiliation: | Tochigi Research Laboratories of Kao Corporation, Ichikai, Haga, Japan. 381128@kastanet.kao.co.jp |
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Abstract: | Substrate specificity of cis-desaturation of alipahtic compounds by resting cells of a mutant, Rhodococcus sp. strain KSM-MT66, was examined. Among substrates tested, the rhodococcal cells were able to convert n-alkanes (C13-C19), 1-chloroalkanes (C16 and C18), ethyl fatty acids (C14-C17) and alkyl (C1-C4) esters of palmitic acid to their corresponding unsaturated products of cis configuration. The products from n-alkanes and 1-chloroalkanes had a double bond mainly at the 9th carbon from their terminal methyl groups, and the products from acyl fatty acids had a double bond mainly at the 6th carbon from their carbonyl carbons. |
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