Target of inhibition by the anti-lipogenic antibiotic cerulenin of sterol synthesis in yeast |
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Authors: | T Ono T Kesado J Awaya S Omura |
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Affiliation: | The Kitasato Institute and Kitasato University 5-9-1, Shirokane, Minato-ku, Tokyo, Japan |
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Abstract: | The antibiotic cerulenin inhibited the incorporation of 14C-acetyl-CoA by 67% at a concentration of 9 × 10?6 M but not that of 14C-HMG-CoA into the non-saponifiable fraction in a cell-free extract of Saccharomyces cerevisiae. Cerulenin markedly inhibited the activity of partially purified HMG-CoA synthase. No inhibition of acetoacetyl-CoA thiolase activity was observed in the same preparation of HMG-CoA synthase. Therefore, cerulenin inhibition of overall sterol synthesis may be accounted for by the specific inhibition of HMG-CoA synthase activity. |
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Keywords: | HMG-CoA β-Hydroxy-β-methylglutaryl Coenzyme-A NSF non-saponifiable fraction ACP acyl carrier protein |
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