Production and partial characterization of extracellular proteinases from <Emphasis Type="Italic">Streptomyces malaysiensis</Emphasis>, isolated from a Brazilian cerrado soil |
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Authors: | Email author" target="_blank">Rodrigo?P?NascimentoEmail author Claudia?M?d’Avila-Levy Rodrigo?F?Souza Marta?H?Branquinha Elba?P?S?Bon Nei?Pereira-Jr Rosalie?R?R?Coelho |
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Institution: | (1) Dep. Microbiologia Geral, Instituto de Microbiologia Prof. Paulo de Góes, CCS, Universidade Federal do Rio de Janeiro (UFRJ), CEP, 21941-590 Rio de Janeiro, Brazil;(2) Dep. Bioquímica, Instituto de Química, CT, UFRJ, Brazil;(3) Escola de Química, CT, UFRJ, Brazil |
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Abstract: | Streptomyces malaysiensis AMT-3, isolated from a Brazilian cerrado soil, showed proteolytic activities detected by gelatin–sodium dodecyl sulfate-polyacrylamide
gel electrophoresis. The optimum proteinase production was obtained when using 2.5% wheat bran and 0.1% yeast extract in the
culture medium, after 5 days incubation at 30°C. The enzymatic complex degraded gelatin optimally at pH 7.0, and under these
conditions eight proteolytic bands (four serine-proteinases and four metaloproteinases), ranging from 20 to 212 kDa, were
detected on the culture supernatant filtrates. In addition, a 35-kDa proteinase was thermostable at 60°C for 120 min. These
results point out to the applicability of gelatin zymograms in the characterization of crude enzymatic complexes. According
to our results, this enzymatic complex could be used for biotechnological applications. |
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Keywords: | Streptomyces malaysiensis Thermostable proteinase Enzyme characterization Wheat bran |
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