Microfilaments Anchor Chloroplasts along the Outer Periclinal Wall in Vallisneria Epidermal Cells through Cooperation of PFR and Photosynthesis |
| |
Authors: | Dong Xia-Jing; Nagai Reiko; Takagi Shingo |
| |
Institution: | Department of Biology, Graduate School of Science, Osaka University Machikaneyama 1-16, Toyonaka, Osaka, 560-0043 Japan |
| |
Abstract: | In the cytoplasmic layer that faces the outer periclinal wallin epidermal cells of leaves of the aquatic angiosperm Vallisneriagigantea Graebner, we examined a possible interrelationshipamong the configuration of microfilaments, chloroplast motility,and anchoring of chloroplasts. In dark-adapted cells, microfilamentsare arranged in a network array. During a 10-min incubationin darkness 10 to 20 min after irradiation with red light (650nm, 0.41 W m2) for 5 min, the number of cells containinga network array decreased substantially while the number ofcells containing microfilaments in a honeycomb array increased.Irradiation with red light rapidly produces an increase in chloroplastmotility, but chloroplast motility declined almost to initiallevels during the 10-min incubation in darkness after the irradiation.Simultaneously, the chloroplasts in these cells became extremelyresistant to centrifugal forces. These effects of red lightwere negated either by far-red light or by the presence of DCMU,and were sensitive to cytochalasin B. It appears, therefore,that microfilaments not only drive the movement of chloroplastsbut also play a crucial role in accumulation of the chloroplastsalong the outer periclinal wall through dynamic changes in theconfiguration under cooperative regulation by PFR and photosynthesis. (Received July 24, 1998; Accepted September 22, 1998) |
| |
Keywords: | |
本文献已被 Oxford 等数据库收录! |
|