Transfer of isolated nuclei into protoplasts ofSaccharomyces cerevisiae |
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Authors: | Lajos Ferenczy Miklós Pesti |
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Institution: | (1) Department of Microbiology, Attila József University, H-6701 Szeged, Hungary;(2) Research Institute, Seed Production and Trading Company, H-6601 Szentes, Hungary |
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Abstract: | Cell nuclei were prepared from protoplasts of an adenine-requiring strain ofSaccharomyces cerevisiae, then purified in a discontinuous sucrose gradient, and applied to protoplasts of a recipient strain auxotrophic for uracil, leucine, and histidine. The transfer of the isolated nuclei into protoplasts was induced with polyethylene glycol. The main products of nuclear transfer in young complemented colonies were heterokaryons giving rise to parental type spontaneuos segregants on nutritionally complete medium. After several passages in minimal medium, however, the prototrophic colonies consisted exclusively of stable heterozygous diploid cells. |
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