Isolation and characterization of α‐(1→3)‐glucan‐degrading bacteria from the gut of Diaperis boleti feeding on Laetiporus sulphureus |
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Authors: | Adrian Wiater,Ma gorzata Pleszczy ska,Ewa Pietrykowska‐Tudruj,Monika Janczarek,Bernard Staniec,Janusz Szczodrak |
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Affiliation: | Adrian Wiater,Małgorzata PleszczyŃska,Ewa Pietrykowska‐Tudruj,Monika Janczarek,Bernard Staniec,Janusz Szczodrak |
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Abstract: | Bacteria degrading α‐(1→3)‐glucan were sought in the gut of fungivorous insects feeding on fruiting bodies of a polypore fungus Laetiporus sulphureus, which are rich in this polymer. One isolate, from Diaperis boleti, was selected in an enrichment culture in the glucan‐containing medium. The bacterium was identified as Paenibacillus sp. based on the results of the ribosomal DNA analysis. The Paenibacillus showed enzyme activity of 4.97 mU/cm3 and effectively degraded fungal α‐(1→3)‐glucan, releasing nigerooligosaccharides and a trace amount of glucose. This strain is the first reported α‐(1→3)‐glucan‐degrading microorganism in the gut microbiome of insects inhabiting fruiting bodies of polypore fungi. |
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Keywords: | α ‐(1→ 3)‐glucanase
Diaperis boleti fungivore
Paenibacillus sp |
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