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Isolation and characterization of polymorphic simple sequence repeat loci in Armillaria ostoyae
Authors:S R H Langrell  B Lung‐Escarmant  S Decroocq
Abstract:A modified hybridization strategy was used to construct a microsatellite enriched library from DNA of Armillaria ostoyae, a serious root pathogen on pine. Sequence characterization of 19 random clones revealed 12 distinct loci harbouring a repetitive motif. Primer design from the flanking regions allowed for their development as polymerase chain reaction based markers. Polymorphic assessment at both the population and global levels revealed levels of variation useful for genetic studies. The level of cross‐species amplification observed with closely related Armillaria species was high, raising the possible exploitation of these primers across the genus.
Keywords:Armillaria  microsatellites  PCR markers  phytopathogenic fungi
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