Plasmid dna transformation of Lactobacillus strains by electropermeabilization |
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| Authors: | Marisa Vescovo F Bosi O Borsani V Bottazzi |
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| Institution: | (1) Istituto di Microbiologia - Università Cattolica, 29100 Piacenza, Italy |
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| Abstract: | Summary Two thermophilic strains of Lactobacillus were transformed by electroporation; L.fermentum with a maximum of frequency of 1×105/ug of plasmid vector pPSC20DNA and 1.4×103/ug pSA3DNA. L.helveticus showed a very low frequency of transformation, from 9 to 26 transformants/ug DNA in all the experiments carried out with both the vectors. While L.fermentum transformants were very stable, in L.helveticus the acquired plasmid was lost after 30–50 generations. |
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