Hybrid respiration in the denitrifying mitochondria of Fusarium oxysporum |
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Authors: | Takaya Naoki Kuwazaki Seigo Adachi Yoshiaki Suzuki Sawako Kikuchi Tomoko Nakamura Hiro Shiro Yoshitsugu Shoun Hirofumi |
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Affiliation: | Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305-8572. |
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Abstract: | Induction of the mitochondrial nitrate-respiration (denitrification) system of the fungus Fusarium oxysporum requires the supply of low levels of oxygen (O(2)). Here we show that O(2) and nitrate (NO(3)(-)) respiration function simultaneously in the mitochondria of fungal cells incubated under hypoxic, denitrifying conditions in which both O(2) and NO(3)(-) act as the terminal electron acceptors. The NO(3)(-) and nitrite (NO(2)(-)) reductases involved in fungal denitrification share the mitochondrial respiratory chain with cytochrome oxidase. F. oxysporum cytochrome c(549) can serve as an electron donor for both NO(2)(-) reductase and cytochrome oxidase. We are the first to demonstrate hybrid respiration in respiring eukaryotic mitochondria. |
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