Monitoring the formation of Maillard reaction products of glucosamine with fibrinogen and human serum albumin using capillary electrophoresis |
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Authors: | Zhang Xinfeng Ma Yue Liu Hong de Sa Paula F G Brown Phyllis R Dain Joel A |
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Affiliation: | Department of Chemistry, University of Rhode Island, Kingston, RI 02881, USA. |
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Abstract: | Formation of Maillard reaction products (MRP) of glucosamine (GlcN) with fibrinogen and human serum albumin (HSA), under simulated physiological conditions, was detected by fluorescence (excitation/emission: 340/420 nm) and UV/Vis (max. 275 nm) spectroscopy. The application of polyacrylamide gel electrophoresis demonstrated the generation of high-molecular-weight fibrinogen and HSA MRP by GlcN. A simple and rapid capillary electrophoresis method was developed to separate MRP formed by the reaction of GlcN with proteins from GlcN autocondensation products. |
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Keywords: | MRP AGE Glucosamine Fibrinogen Human serum albumin Capillary electrophoresis |
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