Comparative characterization of methanotrophic enrichments by serological and molecular methods |
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| Authors: | N. V. Slobodova T. V. Kolganova E. S. Boulygina B. B. Kuznetsov T. P. Tourova I. K. Kravchenko |
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| Affiliation: | (1) Bioengineering Center, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 1, Moscow, 117312, Russia;(2) Winogradsky Institute of Microbiology RAS, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia |
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| Abstract: | Three stable methane-oxidizing enrichment cultures, SB26, SB31, and SB31A, were analyzed by transmission electron microscopy and by serological and molecular techniques. Electron microscopy revealed the presence of both type I and type II methanotrophs in SB31 and SB31A enrichments; only type II methanotrophs were found in SB26 enrichment. Methylosinus trichosporium was detected in all three enrichments by the application of species-specific antibodies. Additionally, Methylocystis echinoides was found in SB26 culture; Methylococcus capsulatus, in SB31 and SB31A; and Methylomonas methanica, in SB31. The analysis with pmoA and nifH gene sequences as phylogenetic markers revealed the presence of Methylosinus/Methylocystis group in all communities. Moreover, the analysis of pmoA sequences revealed the presence of Methylomonas in SB31. Methylocella was detected in SB31 and SB31A enrichments only by nifH analysis. It was concluded that the simultaneous application of different approaches reveals more reliable information on the diversity of methanotrophs. |
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| Keywords: | methanotrophs immunofluorescence pmoA and nifH genes |
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