Somatic Embryogenesis and Plant Regeneration from Leaf Callus of Hordeum vulgare |
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Authors: | MOHANTY, B. D. GHOSH, P. D. |
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Affiliation: | Department of Botany, University of Kalyani Kalyani-741 235, India |
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Abstract: | Explants obtained from the basal portion of leaves of Hordeumvulgare (cv. Karan 92) gave rise to callus when cultured onMurashige and Skoog (MS) basal medium supplemented with 2, 4-dichlorophenoxyaceticacid (2, 4-D). Initially, the callus was friable, shiny-whiteand watery but subsequently some compact, nodular callus appeared.The latter were cultured on MS medium containing 0.05 mg l12, 4-D and 0.1 mg l1 N6-furfurylaminopurine (kinetin),when plantlets were generated. Histological studies showed thatplantlet regeneration occurred by the formation of somatic embryos.The regenerated plants had the normal diploid chromosome number(2n = 14). Hordeum vulgare, barley, somatic embryogenesis, tissue culture, plant regeneration |
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