| 酵母双杂交筛选人乳腺cDNA文库中与Gankyrin相互作用的蛋白 |
| |
| 引用本文： | 顾跃西,梁冰,满江红,李慧艳,李爱玲,张学敏,周涛. 酵母双杂交筛选人乳腺cDNA文库中与Gankyrin相互作用的蛋白[J]. 生物技术通讯, 2009, 20(2): 177-179,186. DOI: 10.3969/j.issn.1009-0002.2009.02.008 |
| |
| 作者姓名： | 顾跃西 梁冰 满江红 李慧艳 李爱玲 张学敏 周涛 |
| |
| 作者单位： | 国家生物医学分析中心,北京,100850 |
| |
| 摘 要： | 目的：应用酵母双杂交技术,筛选与Gankyrin有相互作用的蛋白质。方法：应用酵母双杂交系统,以Gankyrin基因全长为诱饵,在人乳腺cDNA文库中筛选能与Gankyrin相互作用的蛋白质,并运用营养缺陷型培养基和X-α-Gal等实验提供的信息,筛除假阳性克隆。结果：以Gankyrin为诱饵筛选出了5个阳性克隆,对其中一个蛋白质RhoGDI2与Gankyrin进行了免疫共沉淀与GSTpull-down实验验证,证实了它们的相互作用。结论：Gankyrin能与RhoGDI2发生相互作用,在此基础上,可以开展Gankyrin调控肿瘤发生机制的研究。
|
| 关 键 词： | Gankyrin 酵母双杂交 RhoGDI2 免疫共沉淀 GSTpull-down |
Screening of Proteins Interacting with Gankyrin by Yeast Two-Hybrid in Human Breast cDNA Library |
| |
| GU Yue-Xi,LIANG Bing,MAN Jiang-Hong,LI Hui-Yan,LI Ai-Ling,ZHANG Xue-Min,ZHOU Tao. Screening of Proteins Interacting with Gankyrin by Yeast Two-Hybrid in Human Breast cDNA Library[J]. Letters in Biotechnology, 2009, 20(2): 177-179,186. DOI: 10.3969/j.issn.1009-0002.2009.02.008 |
| |
| Authors: | GU Yue-Xi LIANG Bing MAN Jiang-Hong LI Hui-Yan LI Ai-Ling ZHANG Xue-Min ZHOU Tao |
| |
| Affiliation: | ( National Center of Biomedical Analysis, Beijing 100850, China) |
| |
| Abstract: | Objective： To find proteins interacting with Gankyrin by using yeast two-hybrid. Methods： Yeast two-hybrid was applied to screen human breast cDNA library, and Gankyrin as the bait, respectivley. SD nutrient medium and X-αGal were used to exclude the false positive. Results： Five proteins were found to interact with Gankyrin, one of which, RhoGDI2, was confirmed the interaction by Co-IP and GST pull-down. Conclusion： Gankyrin can interact with RhoGDI2, and researches of the mechanisms of Gankyrin as a regulator of tumorigenesis can be carried out based on this interaction. |
| |
| Keywords: | Gankyrin RhoGDI2 GST pull-down |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
