Secretion of Escherichia coli DsbA and DsbC proteins from Brevibacillus choshinensis: stimulation of human epidermal growth factor production |
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| Authors: | Ryoichi Tanaka Masahiro Hashimoto Matsujiro Ishibashi Hiroko Tokunaga Satoru Taura Makoto Mizukami Akira Miyauchi Masao Tokunaga |
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| Institution: | (1) Faculty of Agriculture, Laboratory of Applied and Molecular Microbiology, Japan;(2) Gene Research Center, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan;(3) Department of Research, Development, Higeta Shoyu Co., Choshi, Chiba, 288-8680, Japan |
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| Abstract: | DsbA and DsbC, members of the thioredoxin super-family of redox proteins, which are expressed in the periplasmic space of Escherichia coli, were cloned into and successfully secreted from Brevibacillus choshinensis at  100  g ml–1. Both proteins were active in exchanging disulfide bonds of bovine insulin in vitro. Furthermore, DsbA secreted by B. choshinensis promoted the conversion of non-native human epidermal growth factor to the native form. |
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| Keywords: | Brevibacillus choshinensis Dsb epidermal growth factor insulin secretion |
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